RESEARCH AREAS: anthropology, cell and developmental biology, tissue engineering, experimental morphology and oncology, clinical anatomy, and neuroscience.
METHODS USED: micro preparation, microsurgical techniques, histology and light microscopy, enzyme histochemistry, immunohistochemistry, lectin histochemistry, molecular biology techniques including in situ hybridization, tissue culture, fluorescence microscopy, optical mapping, and basic biochemical methods (ELISA, Western Blot). We use also computer modeling.
LABORATORY SPACE: The labs are located on the premises of the Institute of Anatomy. Additional lab space is found in the BIOCEV in Vestec (collaborative venture between the Charles University and Czech Academy of Sciences). More complex techniqes are performed in collaboration with the Czech Academy of Sciences as well as other national and foreign labs from countries including Germany, USA, France, South Korea, and Singapore.
Studying the skull formation from the perspective of comparative and developmental anatomy using the collections of the Comparative Anatomy Museum of the Institute. Study of intracranial structures, malformations of the facial skeleton and skull base; modeling the soft tissue based on bony landmarks using anthropology approaches combined with CT and CBCT. Study of age-related changes of the face with the aim of identifying people based on skeleton or photographs. Reconstruction of selected specimens from our museum.
Study of molecular mechanisms that influence transformation of neural crest cells; characterization of stem cells of neural crest origin derived from human hair follicles using the chick embryo model and melanoma.
Additional research themes include phenotypic characterization of cells in squamous and glandular epithelia under normal and pathological conditions (basalioma, spinous cells carcinoma of the skin and ductal carcinoma of the pancreas). Epidermal stem cells and epithelia-mesenchymal interactions are studied under both normal conditions as well as wound healing or cancer growth.
Heart development and pathogenesis of heart failure are studied under normal and altered hemodynamic conditions with the aim to elucidate molecular mechanisms governing myocyte proliferation, conduction system differentiation, and factors modulating myocardial fibrosis.
Clinico-anatomical studies are currently focused on development of joints ligaments of the limbs in relation to growth-dependent changes on the skeleton.
Neuroscience research is focused on volumetric studies of the basal ganglia correlating gross anatomy with MRI in patients with Parkinson’s or Alzheimer’s disease, and those after carotid arteries occlusion.
Facial reconstruction based on the skull and subcutaneous tissue with a particular accent on main identifying features. Study of morphology of the upper airways with a focus on the vocal cords. The aim is to differentiate, based upon the architecture and tissue density, the individual components of the vocal apparatus and then digitally reconstruct human larynx as a template for individually tailored prosthetic replacements.
This project is a collaboration between our 3D printing facility and plastination lab and the Institute of Clinical and Experimental Medicine, Institute of Macromolecular Chemistry of the Czech Academy of Sciences and the Faculty of Mechanics and Mechanotronics of the Czech Technical University in Prague.
Selected papers:
Šidlof, P., Švec, J.G., Horáček, J., Veselý, J., Klepáček, I., Havlík, R.: Geometry of human vocal folds and glottal channel for mathematical and biomechanical modeling of voice production. J Biomech. 2008; 41(5):985-95. ISSN: 0021-9290; 1873-2380 (elektronická verze).
Klepáček, I., Naňka, O.: "Bochdalek" skull (syngnathia): CT examination. Brat. Lék. Listy: 110(12):796-801. 2009
Shbat, A., Smrcka, V.: Children´s cranial lesions from Neolithic. Prague Med Rep 2009; 110 (2): 114-119
Klepáček, I., Zedníková-Malá, P.: Bochdalek´s skull: morphology report and reconstruction of face. Forensic Sci Med Pathol. 2012; 8 (4): 451-459
Klepacek I., Jirak D., Smrckova M.D., Janouskova O., Vampola T.: The Human Vocal Fold Layers. Their Delineation Inside Vocal Fold as a Background to Create 3D Digital and Synthetic Glottal Model. J Voice. 2015 Sep 29. pii: S0892-1997(15)00170-8. doi: 10.1016/j.jvoice.2015.08.004
This laboratory studies the epithelial tissues under normal and pathological conditions. We study the interactions between the cancerous and non-cancerous cells in tumor microenvironment. Particular attention is paid to cancer-associated fibroblasts (CAFs) and their products. We focus on factors promoting the inflammatory response (IL-6, IL-8, and TGF-B). These factors appear to be the fundamental “words” in intracellular communication that influence tumor growth and metastasis. Considering the importance of inflammation in healing, tumor microenvironment and fatal viral infections we are looking for common junction points with the goal to better characterize them for ultimate therapeutic modulation. Lab activities are supported by the projects Center of Tumor Ecology and National Institute for Cancer research (funded by the EU).
Selected papers:
Abramenko N, Vellieux F, Tesařová P, Kejík Z, Kaplánek R, Lacina L, Dvořánková B, Rösel D, Brábek J, Tesař A, Jakubek M, Smetana K Jr.: Estrogen Receptor Modulators in Viral Infections Such as SARS−CoV−2: Therapeutic Consequences. Int. J. Mol. Sci. 2021, 22, 6551 (IF 5.923)
Gál P, Brábek J, Holub M, Jakubek M, Šedo A, Lacina L, Strnadová K, Dubový P, Hornychová H, Ryška A, Smetana K Jr.: Autoimmunity, cancer and COVID-19 abnormally activate wound healing pathways: critical role of inflammation. Histochem Cell Biol. 2022 Jul 22:1-20. doi: 10.1007/s00418-022-02140-x. (IF2.531)
Novák Š, Kolář M, Szabó A, Vernerová Z, Lacina L, Strnad H, Šáchová J, Hradilová M, Havránek J, Španko M, Čoma M, Urban L, Kaňuchová M, Melegová N, Gürlich R, Dvořák J, Smetana K Jr, Gál P, Szabo P.: Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts. Cancer Genomics Proteomics 18: 221-243 (2021) (IF 4.069)
Strnadová K, Pfeiferová L, Přikryl P, Dvořánková B, Vlčák E, Frýdlová J, Vokurka M, Novotný J, Šáchová J, Hradilová M, Brábek J, Šmigová J, Rösel D, Smetana K Jr, Kolář M, Lacina L: Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts. Histochem Cell Biol. 157: 153-152 (2022) (IF 4.304)
Talianová V, Kejík Z, Kaplánek R, Veselá K, Abramenko N, Lacina L, Strnadová K, Dvořánková B, Martásek P, Masařík M, Houdová Megová M, Bušek P, Křížová J, Zdražilová L, Hansíková H, Vlčák E, Filimonenko V, Šedo A, Smetana K Jr., Jakubek M: New-Generation Heterocyclic Bis-Pentamethinium Salts as Potential Cytostatic Drugs with Dual IL-6R and Mitochondria-Targeting Activity. Pharmaceutics 2022, 14, 1712. (IF6.525)
Vokurka M, Lacina L, Brábek J, Kolář M, Ng YZ, Smetana, K Jr: Cancer-Associated Fibroblasts Influence the Biological Properties of Malignant Tumours via Paracrine Secretion and Exosome Production. Int. J. Mol. Sci. 23, 964 (2022) (IF 5.924)
We are currently studying the regenerative potential of reptilian heart, development of the cardiac conduction system, and morphogenesis and plasticity of the pectinate muscles in the vertebrate atria. We study the differential response of the adult heart to pressure and volume overload with particular attention to cardiac fibrosis in collaboration with the Institute of Clinical and Experimental Medicine. We are also developing and optimizing new techniques for imaging of the cardiovascular system. This research is supported by the grants of the Czech Science Foundation, Agency for Health-related research, and the Ministry of Education, Youth and Sports.
Selected papers:
Bohuslavova R, Cerychova R, Papousek F, Olejnickova V, Bartos M, Görlach A, Kolar F, Sedmera D, Semenza GL, Pavlinkova G.: HIF-1α is required for development of the sympathetic nervous system. Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13414-13423. doi: 10.1073/pnas.1903510116. Epub 2019 Jun 13.
Kočková R, Línková H, Hlubocká Z, Pravečková A, Polednová A, Súkupová L, Bláha M, Malý J, Honsová E, Sedmera D, Pěnička M.: New Imaging Markers of Clinical Outcome in Asymptomatic Patients with Severe Aortic Regurgitation. J Clin Med. 2019 Oct 11;8(10):1654. doi: 10.3390/jcm8101654. PMID: 31614523
Kvasilova A, Olejnickova V, Jensen B, Christoffels VM, Kolesova H, Sedmera D, Gregorovicova M.: The formation of the atrioventricular conduction axis is linked in development to ventricular septation. J Exp Biol. 2020 Oct 12;223(Pt 19):jeb229278. doi: 10.1242/jeb.229278. PMID: 33046580
Olejnickova V, Kocka M, Kvasilova A, Kolesova H, Dziacky A, Gidor T, Gidor L, Sankova B, Gregorovicova M, Gourdie RG, Sedmera D.: Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle. Int J Mol Sci. 2021 Mar 1;22(5):2475. doi: 10.3390/ijms22052475. PMID: 33804428
Kolesová H, Olejníčková V, Kvasilová A, Gregorovičová M, Sedmera D.: Tissue clearing and imaging methods for cardiovascular development. iScience. 2021 Apr 1;24(4):102387. doi: 10.1016/j.isci.2021.102387. eCollection 2021 Apr 23. PMID: 33981974
Kvasilova A, Gregorovicova M, Olejnickova V, Kolesova H, Sedmera D.: Myocardial development in crocodylians. Dev Dyn. 2022 Dec;251(12):2029-2047. doi: 10.1002/dvdy.527. Epub 2022 Sep 15. PMID: 36045487
Clinic-anatomical studies are focused predominantly on the ligaments of the large joints of the limbs and their relationship to growth-related skeletal changes, as well as growth and development of the cervical spine and possibilities of its osteosynthesis. We are involved also in pioneering selected surgical approaches with the aim to maximally preserve neurovascular structures, both open surgery and ultrasound-guieded procedures. In collaboration with our urogynecology colleagues we are optimizing application of mechanical devices supporting the urethra to restore continence.
Selected papers:
Bartoníček J, Tuček M, Strnad T, Naňka O.: Fractures of the coracoid process - pathoanatomy and classification: based on thirty nine cases with three dimensional computerised tomography reconstructions. Int Orthop. 2020, 45(4): 1009-1015. doi: 10.1007/s00264-020-04634-6.
Fojtík P, Kostlivý K, Bartoníček J, Naňka O.: The fibular notch: an anatomical study. Surg Radiol Anat. 2020 Oct;42(10):1161-1166. doi: 10.1007/s00276-020-02476-w
Strnad T., Bartoníček J., Naňka O., Tuček M.: The coracoglenoid notch: anatomy and clinical signifcance. Surg Radiol Anat. 2021 Jan;43(1):11-17. doi: 10.1007/s00276-020-02527-2.
Štulík J, Geri G, Salavcová L, Barna M, Fojtík P, Naňka O: Pediatric dens anatomy and its implications for fracture treatment: an anatomical and radiological study. Eur Spine J. 2021 Feb;30(2):416-424. doi: 10.1007/s00586-020-06490-9
Bartoníček J., Tuček M., Naňka O.: Poranění lopatky. Maxdorf 2021, 780 stran, 1. vydání, ISBN: 978-80-7345-690-0
Mezian K, Ricci V, Güvener O, Jačisko J, Novotný T, Kara M, Chang KV, Naňka O, Pirri C, Stecco C, Dughbaj M, Jain NB, Özçakar L.: EURO-MUSCULUS/USPRM Dynamic Ultrasound Protocols for (Adult) Hip. Am J Phys Med Rehabil. 2022 Jun 9. doi: 10.1097/PHM.0000000000002061.
Ricci V, Ricci C, Mezian K, Naňka O, Özçakar L.: Trapezius Muscle and the Cutaneous Branches of Spinal Nerves: Sonographic/Anatomic Discussion on Myofascial Pain and Superficial Injections. Pain Med. 2022 Aug 18:pnac125. doi: 10.1093/pm/pnac125. Epub ahead of print. PMID: 35980166
Diego Tavoletti, Ondrej Nanka, Elisabetta Rosano’, Paolo Cerchiara, Elisabetta Cerutti, Luca Pecora: A novel technique of ultrasound-guided lateral cutaneous branch of the iliohypogastric nerve block: a cadaveric study. Acta Anaesthesiologica Scandinavica. 2022, 2022 Sep;66(8):1003-1008, DOI: 10.1111/aas.14110
We continue our long-term collaboration with the Neurology and Radiology departments of the General University Hospital in the area of sleep medicine. We perform volumetric studies of the hippocampus and amygdala using ScanView software on MRI pictures, comparing patients suffering from narcolepsy and cataplexy with healthy individuals. In collaboration with the neurosurgeons from the Central Military Hospital we are studying the course of peripheral nerves on fixed cadavers and device the best surgical approaches. Further collaboration involves the hospital in Usti nad Labem in the area of teaching and clinically-oriented papers.
Selected papers:
NĚMCOVÁ, V., KRÁSENSKÝ, J., KEMLINK, D., PETROVICKÝ, P., VANĚČKOVÁ, M., SEIDL, Z., RULSEH, A., BUŠKOVÁ, J., ŠUSTA, M., ŠONKA, K.: Hippocampal but not amygdalar volume loss in narcolepsy with cataplexy. Neuroendocrinology Letters. 2015, 36(7), 682-688. ISSN 0172-780X. IF = 0.946 (2015)
BARTOŠ, R., NĚMCOVÁ, V., RADOVNICKÝ, T., SEJKOROVÁ, A., MALUCELLI, A., ORLICKÝ, M., TREBICKÝ, F., SAMEŠ, M.: Gliomy limbického a paralimbického systému, technika a výsledky resekcí. Česká a slovenská neurologie a neurochirurgie. 2016, 79(2), 131-147. ISSN 1210-7859. IF = 0.368 (2016)
BRABEC, J., BULDOV, S. V., CITA, S., CITOVÁ, I., NĚMCOVÁ, V., NOVÁKOVÁ, Z., SMAZALOVÁ, J., KACHLÍK, D.: Lidské tělo. 2 vyd. Praha: Knižní klub, 2016. 288 s. ISBN 978-80-242-5301-5.
KAISER, R., ASTL, J., BELŠAN, T., BENEŠ, V., DVOŘÁKOVÁ, P., HRABAL, P., KRAMÁŘ, F., KUKAČKOVÁ, M., MASOPUST, V., NĚMCOVÁ, V., ŘÍHA, M., SÁDLOVÁ, T., SKALKOVÁ, I., TUČEK, M., TYLL, T., VONDRÁČKOVÁ, D.: Chirurgie hlavových a periferních nervů s atlasem přístupů. 1 vyd. Praha: Grada, 2016. 212 s. ISBN 978-80-247-5808-4.
VACHATA, P., SAMEŠ, M., BETKA, J., BOJAR, M., CEÉ, J., CIHLÁŘ, F., FRAJER, L., HUMHEJ, I., CHOVANEC, M., JELÍNKOVÁ, V., KLENER, J., KOLÁŘ, P., KOZÁK, J., KUFA, R., LIŠČÁK, R., NĚMCOVÁ, V., PETROVICKÝ, P., SKŘIVAN, J., TICHÝ, M., TOMÁŠ, R., VAVERKA, M., VLASÁK, A., ZÁBRODSKÝ, M., ZIKMUND, L., ZOLAL, A., ZVĚŘINA, E.: Lícní nerv: anatomie, patologie, léčba. 1 vyd. Praha: Mladá fronta, 2016. 381 s. ISBN 978-80-204-3252-0.
ŠUSTA, M., NĚMCOVÁ, V., BIZIK, G., ŠONKA, K.: Emotion stimulus processing in narcolepsy with cataplexy. Journal of Sleep Research. 2017, 26(1), 30-37. ISSN 0962-1105. DOI: 10.1111/jsr.12444 IF = 3.259 (2016)
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